BCL9 Polyclonal antibody

BCL9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22947-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

Bcl-9, Protein legless homolog, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22947-1-AP targets BCL9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human
文献引用反应性human, mouse
免疫原 BCL9 fusion protein Ag19158 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell CLL/lymphoma 9
别名 Bcl-9, Protein legless homolog, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein
计算分子量 1426 aa, 149 kDa
观测分子量 149 kDa
GenBank蛋白编号BC116451
基因名称 BCL9
Gene ID (NCBI) 607
RRIDAB_2879189
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00512
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

实验方案

Product Specific Protocols
WB protocol for BCL9 antibody 22947-1-APDownload protocol
IHC protocol for BCL9 antibody 22947-1-APDownload protocol
IF protocol for BCL9 antibody 22947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bioorg Chem

Discovery of 2-(isoxazol-5-yl)phenyl 3,4-dihydroxybenzoate as a potential inhibitor for the Wnt/β-catenin pathway

Authors - Yongxi Dong
human,mouseWB,IHC

Reprod Biol Endocrinol

Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.

Authors - Xi Wang
humanWB,IF,IP

J Biol Chem

USP9X-mediated deubiquitination of B cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.

Authors - Zesen Shang
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