验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11740-1-AP targets BCL7B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2333 Product name: Recombinant human BCL7B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC000956 Sequence: MSGRSVRAETRSRAKDDIKKVMAAIEKVRKWEKKWVTVGDTSLRIFKWVPVTDSKEKEKSKSNSSAAREPNGFPSDASANSSLLLEFQDENSNQSSVSDVYQLKVDSSTNSSPSPQQSESLSPAHTSDFRTDDSQPPTLGQEILEEPSLPSSEVADEPPTLTKEEPVPLETQVVEEEEDSGAPPLKRFCVDQPTVPQTASES 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | B-cell CLL/lymphoma 7B |
| 别名 | B cell CLL/lymphoma 7B |
| 计算分子量 | 22 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC000956 |
| 基因名称 | BCL7B |
| Gene ID (NCBI) | 9275 |
| RRID | AB_2063596 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BQE9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for BCL7B antibody 11740-1-AP | Download protocol |
| IHC protocol for BCL7B antibody 11740-1-AP | Download protocol |
| WB protocol for BCL7B antibody 11740-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro. | ||
Aging (Albany NY) S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway. | ||
Sci Rep The SWI/SNF subunit Bcl7a contributes to motor coordination and Purkinje cell function.
| ||
Life Sci Alliance BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression
| ||
Exp Hematol Bcl7b and Bcl7c Subunits of BAF Chromatin Remodeling Complexes are Largely Dispensable for Hematopoiesis |
