验证数据展示
经过测试的应用
Positive WB detected in | Raji cells |
Positive IP detected in | Raji cells |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:3000-1:12000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21187-1-AP targets BCL6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | BCL6 fusion protein Ag15519 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | B-cell CLL/lymphoma 6 |
别名 | BCL-6, BCL-5, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein |
计算分子量 | 706 aa, 79 kDa |
观测分子量 | 79-95 kDa |
GenBank蛋白编号 | BC150184 |
基因名称 | BCL6 |
Gene ID (NCBI) | 604 |
RRID | AB_2878827 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P41182 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.
实验方案
Product Specific Protocols | |
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WB protocol for BCL6 antibody 21187-1-AP | Download protocol |
IHC protocol for BCL6 antibody 21187-1-AP | Download protocol |
IP protocol for BCL6 antibody 21187-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice. | ||
Respir Res Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial-mesenchymal transition during radiation-induced pulmonary fibrosis. | ||
Sci Rep Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine. | ||
Front Cell Dev Biol Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma | ||
Front Cell Dev Biol The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis. | ||
Front Oncol Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney. |