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BCL6 Polyclonal antibody

BCL6 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 21187-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IP, ELISA and More (1)

BCL-6, BCL-5, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inRaji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21187-1-AP targets BCL6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human
文献引用反应性human, mouse
免疫原 BCL6 fusion protein Ag15519 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell CLL/lymphoma 6
别名 BCL-6, BCL-5, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein
计算分子量 706 aa, 79 kDa
观测分子量 79-95 kDa
GenBank蛋白编号BC150184
基因名称 BCL6
Gene ID (NCBI) 604
RRIDAB_2878827
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41182
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

实验方案

Product Specific Protocols
WB protocol for BCL6 antibody 21187-1-APDownload protocol
IHC protocol for BCL6 antibody 21187-1-APDownload protocol
IP protocol for BCL6 antibody 21187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Nat Commun

A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.

Authors - Christine Quach
humanWB

Respir Res

Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial-mesenchymal transition during radiation-induced pulmonary fibrosis.

Authors - Ziyan Yan
mouseWB

Sci Rep

Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.

Authors - Yanhong Shi
humanWB,IP

Front Cell Dev Biol

Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma

Authors - Mayuko Nishi
humanIHC

Front Cell Dev Biol

The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis.

Authors - Zhengyan Chang
humanIHC

Front Oncol

Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.

Authors - Shuang Wen
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