验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse heart tissue, HepG2 cells, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16612-1-AP targets BCL2L13 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9841 Product name: Recombinant human BCL2L13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC003032 Sequence: MLLELTRRGQEPLSALLQFGVTYLEDYSAEYIIQQGGWGTVFSLESEEEEYPGITAEDSNDIYILPSDNSGQVSPPESPTVTTSWQSESLPVSLSASQSWHTESLPVSLGPESWQQIAMDPEEVKSLDSNGAGEKSENNSSNSDIVHVEKEEVPEGMEEAAVASVVLPARELQEALPEAPAPLLPHITATSLLGTREPDTEVITVEKSSPATSLFVELDEEEVKAATTEPTEVEEVVPALEPTETLLSEKEINAREESLVEELSPASEKKPVPPSEGKSRLSPAGEMKPMPLSEGKSILL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BCL2-like 13 (apoptosis facilitator) |
| 别名 | BCL2L13, MIL1, Protein Mil1 |
| 计算分子量 | 485 aa, 53 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC003032 |
| 基因名称 | BCL2L13 |
| Gene ID (NCBI) | 23786 |
| RRID | AB_1850928 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXK5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BCL2L13 antibody 16612-1-AP | Download protocol |
| IP protocol for BCL2L13 antibody 16612-1-AP | Download protocol |
| WB protocol for BCL2L13 antibody 16612-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites | ||
Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy | ||
Nat Commun Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation. | ||
Autophagy BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. | ||
Proc Natl Acad Sci U S A Bcl2L13 is a ceramide synthase inhibitor in glioblastoma.
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