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验证数据展示

  • WB analysis using 80313-1-RR

    WB analysis using 80313-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80313-1-RR (BCL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 80313-1-RR

    IHC staining of human tonsillitis using 80313-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human tonsillitis using 80313-1-RR

    IHC staining of human tonsillitis using 80313-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL2 antibody included
  • IF Staining of human tonsillitis using 80313-1-RR

    IF Staining of human tonsillitis using 80313-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using BCL2 antibody (80313-1-RR, Clone: 3O5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 80313-1-RR

    Affinity and Kinetic Characterization of 80313-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80313-1-RR against Human BCL2 were performed. The affinity constant is 5.06 nM.

BCL2 Recombinant antibody

BCL2 Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 80313-1-RR

产品说明书

CloneNo. 3O5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

BCL-2, 3O5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells, THP-1 cells, HL-60 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80313-1-RR targets BCL2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 BCL2 fusion protein Ag3508 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 2
别名 BCL-2, 3O5
计算分子量 26 kDa
观测分子量26 kDa
GenBank蛋白编号BC027258
基因名称 BCL2
Gene ID (NCBI) 596
RRIDAB_2918886
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10415
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).

实验方案

Product Specific Protocols
WB protocol for BCL2 antibody 80313-1-RRDownload protocol
IHC protocol for BCL2 antibody 80313-1-RRDownload protocol
IF protocol for BCL2 antibody 80313-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols