• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83309-1-RR (same clone as 83309-1-PBS)

    WB analysis using 83309-1-RR (same clone as 83309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83309-1-RR (BCDIN3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83309-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83309-1-RR (same clone as 83309-1-PBS)

    FC experiment of HeLa using 83309-1-RR (same clone as 83309-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BCDIN3D Recombinant antibody (83309-1-RR, Clone:240152A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83309-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00333-1

    Cytometric bead array standard curve of MP00333-1

    Cytometric bead array standard curve of MP00333-1, BCDIN3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83309-1-PBS. Detection antibody: 83309-3-PBS. Standard: Ag15613. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00333-3

    Cytometric bead array standard curve of MP00333-3

    Cytometric bead array standard curve of MP00333-3, BCDIN3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83309-1-PBS. Detection antibody: 83309-4-PBS. Standard: Ag15613. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83309-1-PBS

    Affinity and Kinetic Characterization of 83309-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83309-1-PBS against Human BCDIN3D was performed. The affinity constant is 3.04 nM.

  • Affinity and Kinetic Characterization of 83309-1-RR

    Affinity and Kinetic Characterization of 83309-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83309-1-RR against Human BCDIN3D were performed. The affinity constant is 2.38 nM.

BCDIN3D Recombinant antibody, PBS Only (Capture)

BCDIN3D Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83309-1-PBS

产品说明书

CloneNo. 240152A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

RNA 5'-monophosphate methyltransferase, EC:2.1.1.-, BCDIN3 domain-containing protein, 240152A9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83309-1-PBS targets BCDIN3D as part of a matched antibody pair:

MP00333-1: 83309-1-PBS capture and 83309-3-PBS detection (validated in Cytometric bead array)

MP00333-3: 83309-1-PBS capture and 83309-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 BCDIN3D fusion protein Ag15613 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCDIN3 domain containing
别名 RNA 5'-monophosphate methyltransferase, EC:2.1.1.-, BCDIN3 domain-containing protein, 240152A9
计算分子量 292 aa, 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC053560
基因名称 BCDIN3D
Gene ID (NCBI) 144233
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z5W3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCDIN3D (BCDIN3 domain containing RNA methyltransferase), it is expected to be located in cytoplasm, which is ubiquitous expressed in prostate, lymph node and skeletal muscle. This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. The molecular weight of BCDIN3D is 33 kDa.