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BCAT2 Monoclonal antibody

BCAT2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A5

Cat No : 68586-1-Ig

Print datasheet

Synonyms

BCAT2, ECA40, Placental protein 18, PP18

验证数据展示

  • WB analysis using 68586-1-Ig

    WB analysis using 68586-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68586-1-Ig (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inA431 cells, HCT 116 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, mouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68586-1-Ig targets BCAT2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse
Immunogen BCAT2 fusion protein Ag9595 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name branched chain aminotransferase 2, mitochondrial
Synonyms BCAT2, ECA40, Placental protein 18, PP18
Calculated Molecular Weight 44 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC001900
Gene Symbol BCAT2
Gene ID (NCBI) 587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15382
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

实验方案

Product Specific Protocols
WB protocol for BCAT2 antibody 68586-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols