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验证数据展示

  • WB analysis using 28622-1-AP

    WB analysis using 28622-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28622-1-AP (BCAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28622-1-AP

    IHC staining of human liver cancer using 28622-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BCAT1 Polyclonal antibody

BCAT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28622-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat and More (2)

应用

WB, IHC, ELISA

别名

BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inC6 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28622-1-AP targets BCAT1 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag28998

Product name: Recombinant human BCAT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-115 aa of BC033864

Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 branched chain aminotransferase 1, cytosolic
别名 BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39
计算分子量 320 aa, 36 kDa
观测分子量 43-48 kDa
GenBank蛋白编号BC033864
基因名称 BCAT1
Gene ID (NCBI) 586
RRIDAB_2881183
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54687
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

实验方案

Product Specific Protocols
WB protocol for BCAT1 antibody 28622-1-APDownload protocol
IHC protocol for BCAT1 antibody 28622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Oncol Rep

BCAT1 overexpression regulates proliferation and c‑Myc/GLUT1 signaling in head and neck squamous cell carcinoma.

Authors - Hongming Wang
View Article
mouseWB,IHC

Biochim Biophys Acta Mol Basis Dis

Targeted inhibition of branched-chain amino acid metabolism drives apoptosis of glioblastoma by facilitating ubiquitin degradation of Mfn2 and oxidative stress

Authors - Zhuo Lu
View Article