• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of HeLa using 13640-1-AP (same clone as 13640-1-PBS)

    WB analysis of HeLa using 13640-1-AP (same clone as 13640-1-PBS)

    WB result of BCAT1/ECA39 antibody (13640-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAT1/ECA39 transfected HeLa cells. This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • WB analysis using 13640-1-AP (same clone as 13640-1-PBS)

    WB analysis using 13640-1-AP (same clone as 13640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13640-1-AP (same clone as 13640-1-PBS)

    IHC staining of mouse brain using 13640-1-AP (same clone as 13640-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13640-1-AP (BCAT1/ECA39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13640-1-AP (same clone as 13640-1-PBS)

    IF Staining of HepG2 using 13640-1-AP (same clone as 13640-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCAT1 antibody (13640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13640-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BCAT1 Polyclonal antibody, PBS Only

BCAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13640-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

BCAT1/ECA39, Branched-chain-amino-acid aminotransferase, cytosolic, EC:2.6.1.42, ECA39, MECA39

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

13640-1-PBS targets BCAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4574

Product name: Recombinant human BCAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC033864

Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 branched chain aminotransferase 1, cytosolic
别名 BCAT1/ECA39, Branched-chain-amino-acid aminotransferase, cytosolic, EC:2.6.1.42, ECA39, MECA39
计算分子量 320 aa, 36 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC033864
基因名称 BCAT1
Gene ID (NCBI) 586
RRIDAB_2063569
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54687
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCAT1, also named branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine, and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149). The 13640-1-AP antibody detects the bands around 43-45 kDa for different isoforms and higher molecular weight for phosphorylation modification of this protein.

||
New chat

Able™

正在加载，请稍候...