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验证数据展示

  • WB analysis using 10402-1-AP

    WB analysis using 10402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10402-1-AP (BCAS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 10402-1-AP

    WB analysis of A431 using 10402-1-AP

    WB result of BCAS3 antibody (10402-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAS3 transfected A431 cells.

  • Featured Product
  • KD/KO验证

BCAS3 Polyclonal antibody

BCAS3 Polyclonal Antibody for WB, ELISA
Cat No. 10402-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

BCAS3, GAOB1, MAAB, Protein Maab1, Rudhira

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, A431 cells, HeLa cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10402-1-AP targets BCAS3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BCAS3 fusion protein Ag0646 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 breast carcinoma amplified sequence 3
别名 BCAS3, GAOB1, MAAB, Protein Maab1, Rudhira
计算分子量 99 kDa
观测分子量 74 kDa
GenBank蛋白编号BC001250
基因名称 BCAS3
Gene ID (NCBI) 54828
RRIDAB_10897181
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6U6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rudhira/BCAS3 (Breast Cancer Amplified Sequence 3) is a conserved protein expressed in the embryonic vasculature and malignant tumors. Rudhira is a predicted WD40 domain protein. During mouse development Rudhira is expressed in erythropoietic and angiogenic precursors but not in their differentiated progeny. This transient expression of Rudhira is also seen in normal adult angiogenesis. BCAS3, the human ortholog of Rudhira is 98% identical and the gene maps to a breakpoint of hematological neoplasms on chromosome 17q23.1. BCAS3 is also expressed in vascular development in embryoid bodies in vitro and was originally identified as a gene over-expressed in breast cancers. It is reported to be a target of metastasis associated protein-1 (MTA-1). Further BCAS3 expression is also upregulated in grade III and IV glioblastomas where it is misexpressed in the tumor cells.

实验方案

Product Specific Protocols
WB protocol for BCAS3 antibody 10402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols