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验证数据展示

  • WB analysis using 68003-1-Ig (same clone as 68003-1-PBS)

    WB analysis using 68003-1-Ig (same clone as 68003-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68003-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 68003-1-Ig (same clone as 68003-1-PBS)

    WB analysis of LNCaP using 68003-1-Ig (same clone as 68003-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68003-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68003-1-Ig (same clone as 68003-1-PBS)

    IF Staining of HeLa using 68003-1-Ig (same clone as 68003-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCAS2 antibody (68003-1-Ig, Clone: 2A12G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68003-1-PBS in a different storage buffer formulation.

BCAS2 Monoclonal antibody, PBS Only

BCAS2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68003-1-PBS

产品说明书

CloneNo. 2A12G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Spliceosome-associated protein SPF 27, Pre-mRNA-splicing factor SPF27, Pre mRNA splicing factor SPF27, DAM1, Breast carcinoma-amplified sequence 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68003-1-PBS targets BCAS2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21309

Product name: Recombinant human BCAS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-225 aa of BC005285

Sequence: MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 breast carcinoma amplified sequence 2
别名 Spliceosome-associated protein SPF 27, Pre-mRNA-splicing factor SPF27, Pre mRNA splicing factor SPF27, DAM1, Breast carcinoma-amplified sequence 2
计算分子量 26 kDa
观测分子量28 kDa
GenBank蛋白编号BC005285
基因名称 BCAS2
Gene ID (NCBI) 10286
RRIDAB_2918750
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75934
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Breast carcinoma amplified sequence 2 (BCAS2) is preferentially known as pre-mRNA splicing factor SPF27 and was originally characterized as an up-regulated gene by amplification in human breast cancer cells. BCAS2 has been shown to be involved in DNA damage repair through the replication protein A (RPA) complex.

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