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BCAS1 Polyclonal antibody

BCAS1 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25776-1-AP

Print datasheet

Synonyms

AIBC1, BCAS1, NABC1

验证数据展示

  • WB analysis of NIH/3T3 using 25776-1-AP

    WB analysis of NIH/3T3 using 25776-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25776-1-AP (BCAS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 25776-1-AP

    IF Staining of MCF-7 using 25776-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 25776-1-AP (BCAS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells, MCF-7 cells
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25776-1-AP is BCAS1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse
Immunogen BCAS1 fusion protein Ag22760 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name breast carcinoma amplified sequence 1
Synonyms AIBC1, BCAS1, NABC1
Calculated Molecular Weight 584 aa, 62 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC126348
Gene Symbol BCAS1
Gene ID (NCBI) 8537
RRIDAB_2880235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75363
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BCAS1 antibody 25776-1-APDownload protocol
IF protocol for BCAS1 antibody 25776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols