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验证数据展示

  • WB analysis of NIH/3T3 using 25776-1-AP

    WB analysis of NIH/3T3 using 25776-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25776-1-AP (BCAS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 25776-1-AP

    IHC staining of human stomach using 25776-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25776-1-AP (BCAS1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 25776-1-AP

    IF Staining of MCF-7 using 25776-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using BCAS1 antibody (25776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

BCAS1 Polyclonal antibody

BCAS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25776-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

NABC1, Breast carcinoma-amplified sequence 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, MCF-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25776-1-AP targets BCAS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22760

Product name: Recombinant human BCAS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-289 aa of BC126348

Sequence: MGNQMSVPQRVEDQENEPEAETYKDNASALNGVPVVVSTHTVQHLEEVDLGISVKTDNVATSSPETTEISAVADANGKNLGKEAKPEAPAAKSRFFLMLSRPVPGRTGDQAADSSLGSVKLDVSSNKAPANKDPSESWTLPVAAGPGQDTDKTPGHAPAQDKALSAARDPTLLPPETGGAGGEAPSKPKDSSFFDKFFKLDKGQEKVPGDSQQEAKRAEHQDKVDEVPGLSGQSDDVPAGKDIVDGKEKEGQELGTADCSVPGDPEGLETAKDDSQAAAIAENNNSIMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 breast carcinoma amplified sequence 1
别名 NABC1, Breast carcinoma-amplified sequence 1
计算分子量 584 aa, 62 kDa
观测分子量 68 kDa
GenBank蛋白编号BC126348
基因名称 BCAS1
Gene ID (NCBI) 8537
RRIDAB_2880235
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75363
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for BCAS1 antibody 25776-1-APDownload protocol
IHC protocol for BCAS1 antibody 25776-1-APDownload protocol
WB protocol for BCAS1 antibody 25776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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