验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tissue, human brain tissue, human lung tissue, human placenta tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17254-1-AP targets BBX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | mouse, rat, zebrafish |
| 免疫原 |
CatNo: Ag10691 Product name: Recombinant human BBX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 249-597 aa of BC012837 Sequence: KPEDSDCHRKIETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRSSGKGNSSDHEGCWNEESWTFSQSGTSGSKKFKKTKPKEDCLLGSAKLDEEFEKKFNSLPQYSPVTFDRKCVPVPRKKKKTGNVSSEPTKTSKGPFQSQKKNLFHKIVSKYKHKKEKPNVPEKGSGDKWSNKQLFLDAIHPTEAIFSEDRNTMEPVHKVKNIPSIFNTPEPTTTQEPLVGSQKRKARKTKITHLVRTADGRVSPAGGTLDDKPKEQLQRSLPKATETDCNDKCSHNTEVGETRSSTPEMPAVSAFFSLAALAEVAAMENVHRGQRSTPLTHDGQPKEMPQAPVLISCADQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | bobby sox homolog (Drosophila) |
| 别名 | BBX, Bobby sox homolog, bobby sox homolog (Drosophila), HBP2, HMG box containing protein 2, HSPC339, MDS001 |
| 计算分子量 | 941 aa, 105 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC012837 |
| 基因名称 | BBX |
| Gene ID (NCBI) | 56987 |
| RRID | AB_2065285 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WY36 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BBX antibody 17254-1-AP | Download protocol |
| IP protocol for BBX antibody 17254-1-AP | Download protocol |
| WB protocol for BBX antibody 17254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Mol Med The transcription factor BBX regulates phosphate homeostasis through the modulation of FGF23 | ||
Biol Reprod PiggyBac Transposon-Mediated Mutagenesis in Rats Reveals a Crucial Role of Bbx in Growth and Male Fertility.
| ||
Dev Genes Evol Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic. | ||
Dev Comp Immunol Discovery of BbX transcription factor in the patagonian blennie: Exploring expression changes following combined bacterial and thermal stress exposure |
