验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18961-1-AP targets BBS7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | BBS7 fusion protein Ag4601 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Bardet-Biedl syndrome 7 |
| 别名 | Bardet Biedl syndrome 7, BBS2 like protein 1, BBS2L1, BBS7 |
| 计算分子量 | 715 aa, 80 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC032691 |
| 基因名称 | BBS7 |
| Gene ID (NCBI) | 55212 |
| RRID | AB_2064998 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IWZ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BBS7 antibody 18961-1-AP | Download protocol |
| IHC protocol for BBS7 antibody 18961-1-AP | Download protocol |
| IP protocol for BBS7 antibody 18961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. | ||
PLoS Genet A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened. | ||
PLoS Genet The Centriolar Satellite Protein AZI1 Interacts with BBS4 and Regulates Ciliary Trafficking of the BBSome. | ||
PLoS Genet BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment. | ||
Hum Mol Genet Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome. | ||
FASEB J Rhodopsin mislocalization drives ciliary dysregulation in a novel autosomal dominant retinitis pigmentosa knock-in mouse model |
