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验证数据展示

  • WB analysis of rat brain using 12676-1-AP (same clone as 12676-1-PBS)

    WB analysis of rat brain using 12676-1-AP (same clone as 12676-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 cells using 12676-1-AP (same clone as 12676-1-PBS)

    IF Staining of hTERT-RPE1 cells using 12676-1-AP (same clone as 12676-1-PBS)

    IF result (cytoplasm and the base of cilia stain) of anti-BBS3 (12676-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12676-1-AP (same clone as 12676-1-PBS)

    IHC staining of human kidney using 12676-1-AP (same clone as 12676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12676-1-AP (BBS3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12676-1-AP (same clone as 12676-1-PBS)

    IHC staining of human kidney using 12676-1-AP (same clone as 12676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12676-1-AP (BBS3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 12676-1-AP (same clone as 12676-1-PBS)

    IP experiment of rat brain using 12676-1-AP (same clone as 12676-1-PBS)

    IP result of anti-BBS3 (IP:12676-1-AP, 3ug; Detection:12676-1-AP 1:600) with rat brain tissue lysate 5200ug. This data was developed using the same antibody clone with 12676-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BBS3 Polyclonal antibody, PBS Only

BBS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12676-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12676-1-PBS targets BBS3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原

CatNo: Ag3363

Product name: Recombinant human BBS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC024239

Sequence: MGLLDRLSVLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQNILPTIGFSIEKFKSSSLSFTVFDMSGQGRYRNLWEHYYKEGQAIIFVIDSSDRLRMVVAKEELDTLLNHPDIKHRRIPILFFANKMDLRDAVTSVKVSQLLCLENIKDKPWHICASDAIKGEGLQEGVDWLQDQIQTVKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 6
别名
计算分子量 186 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC024239
基因名称 ARL6/BBS3
Gene ID (NCBI) 84100
RRIDAB_2058675
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0F7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADP-ribosylation factor-like protein 6 (ARL6), belongs to a small GTPase superfamily, is involved in membrane protein trafficking at the base of the ciliary organelle. ARL6 is also named Bardet-Biedl syndrome 3 protein, because defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3), which is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. ARL6 can interact with many ARL6 interacting proteins and form BBSome complex with other BBS proteins including BBS1, BBS2, BBS4 and so on.

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