|Positive WB detected in||mouse testis tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IF detected in||hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11188-2-AP targets BBS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||IF, IP, WB, ELISA|
|Cited Applications||IF, IHC, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Immunogen||BBS2 fusion protein Ag1662|
|Host / Isotype||Rabbit / IgG|
|Full Name||Bardet-Biedl syndrome 2|
|Synonyms||Bardet Biedl syndrome 2, BBS, BBS2|
|Calculated molecular weight||80 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC014140|
|Gene ID (NCBI)||583|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.
The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.
Cell Stem Cell
Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression.
J Cell Biol
Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.
Brain Struct Funct
A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice.
Hum Mol Genet
Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.
Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.