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验证数据展示

  • WB analysis using 66518-1-Ig

    WB analysis using 66518-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66518-1-Ig

    IHC staining of human liver cancer using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66518-1-Ig

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66518-1-Ig

    IHC staining of mouse brain using 66518-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MKS1 Monoclonal antibody

MKS1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66518-1-Ig

产品说明书

CloneNo. 6A8B11

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, rat, mouse

应用

WB, IHC, ELISA

别名

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNCCIT cells, pig brain tissue, HEK-293 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66518-1-Ig targets MKS1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, pig, rat, mouse
免疫原

CatNo: Ag9504

Product name: Recombinant human BBS13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-321 aa of BC010061

Sequence: MTTAASEVPSFLVERMANVRRRRQDRRGMEGGILKSRIVTWEPSEEFVRNNHVINTPLQTMHIMADLGPYKKLGYKKYEHVLCTLKVDSNGVITVKPDFTGLKGPYRIETEGEKQELWKYTIDNVSPHAQPEEEERERRVFKDLYGRHKEYLSSLVGTDFEMTVPGALRLFVNGEVVSAQGYEYDNLYVHFFVELPTAHWSSPAFQQLSGVTQTCTTKSLAMDKVAHFSYPFTFEAFFLHEDESSDALPEWPVLYCEVLSLDFWQRYRVEGYGAVVLPATPGSHTLTVSTWRPVELGTVAELRRFFIGGSLELEDLSYVRI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Meckel syndrome, type 1
别名 BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1
计算分子量 559 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC010061
基因名称 MKS1
Gene ID (NCBI) 54903
RRIDAB_2881881
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NXB0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MKS1 antibody 66518-1-IgDownload protocol
IHC protocol for MKS1 antibody 66518-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols