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验证数据展示

  • WB analysis using 66679-1-Ig

    WB analysis using 66679-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human liver cancer using 66679-1-Ig

    IHC staining of human liver cancer using 66679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 66679-1-Ig

    WB analysis using 66679-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

BBS10 Monoclonal antibody

BBS10 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66679-1-Ig

产品说明书

CloneNo. 4C6A10

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, ELISA

别名

Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNCI-H1299 cells, NCCIT cells, L02 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66679-1-Ig targets BBS10 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag17960

Product name: Recombinant human BBS10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 573-723 aa of BC013795

Sequence: MLPVSCKLPNMGTSQSYLSSSMPAGCVLPVGGNFEILLHYYLLNYAKKCHQSEETMVSMIIANALLGIPKVLYKSKTGKYSFPHTYIRAVHALQTNQPLVSSQTGLESVMGKYQLLTSVLQCLTKILTIDMVITVKRHPQKVHNQDSEDEL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Bardet-Biedl syndrome 10
别名 Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560
计算分子量 81 kDa
观测分子量 81 kDa
GenBank蛋白编号BC013795
基因名称 BBS10
Gene ID (NCBI) 79738
RRIDAB_2882033
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TAM1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID: 20080638, 19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

实验方案

Product Specific Protocols
IHC protocol for BBS10 antibody 66679-1-IgDownload protocol
WB protocol for BBS10 antibody 66679-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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