验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse brain tissue, mouse heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
21118-1-AP targets BBS1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag15460 Product name: Recombinant human BBS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 105-463 aa of BC047642 Sequence: SCLVLGTENKELLVLDPEAFTILAKMSLPSVPVFLEVSGQFDVEFRLAAACRNGNIYILRRDSKHPKYCIELSAQPVGLIRVHKVLVVGSTQDSLHGFTHKGKKLWTVQMPAAILTMNLLEQHSRGLQAVMAGLANGEVRIYRDKALLNVIHTPDAVTSLCFGRYGREDNTLIMTTRGGGLIIKILKRTAMFVEGGSEVGPPPAQAMKLNVPRKTRLYVDQTLREREAGTAMHRAFQTDLYLLRLRAARAYLQALESSLSPLSTTAREPLKLHAVVQGLGPTFKLTLHLQNTSTTRPVLGLLVCFLYNEALYSLPRAFFKVPLLVPGLNYPLETFVESLSNKGISDIIKVGPALVPRGR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Bardet-Biedl syndrome 1 |
| 别名 | Bardet Biedl syndrome 1, BBS1, BBS2 like protein 2, BBS2L2 |
| 计算分子量 | 593 aa, 65 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC047642 |
| 基因名称 | BBS1 |
| Gene ID (NCBI) | 582 |
| RRID | AB_2878814 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NFJ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for BBS1 antibody 21118-1-AP | Download protocol |
| WB protocol for BBS1 antibody 21118-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. | ||
PLoS Biol Melanocortin 1 receptor mediates melanin production by interacting with the BBSome in primary cilia |
