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验证数据展示

  • WB analysis using 24337-1-AP (same clone as 24337-1-PBS)

    WB analysis using 24337-1-AP (same clone as 24337-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24337-1-AP (BBOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

    IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

    IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

BBOF1 Polyclonal antibody, PBS Only

BBOF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24337-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24337-1-PBS targets BBOF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19302

Product name: Recombinant human C14orf45 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 12-229 aa of BC104981

Sequence: LQQHAMIENQAGQVEIDKLQHLLQMKDREMNRVKKLAKNILDERTEVERFFLDALHQVKQQILISRKHYKQIAQAAFNLKMRAACTGRTEYPKIRTFDGREHSTNSVNQDLLEAEKWTHIEGNVDIGDLTWEQKEKVLRLLFAKMNGCPSRKYNQSSRPPVPDYVVSDSGETKEFGDESKLQDKIFITQQIAISDSSGEVVLPTIPKEPQESDTGTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 45
别名
计算分子量 529 aa, 62 kDa
观测分子量62 kDa
GenBank蛋白编号BC104981
基因名称 BBOF1
Gene ID (NCBI) 80127
RRIDAB_3669433
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8ND07
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Basal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates. Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis.

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