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验证数据展示

  • WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

    WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAX antibody included
  • IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAX antibody included
  • WB analysis of HeLa using 60267-1-Ig (same clone as 60267-1-PBS)

    WB analysis of HeLa using 60267-1-Ig (same clone as 60267-1-PBS)

    WB result of BAX antibody (60267-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAX transfected HeLa cells. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

    IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

    IP result of anti-BAX (IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • IHC staining of human rectal cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    IHC staining of human rectal cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAX antibody included
  • IF Staining of MCF-7 using 60267-1-Ig (same clone as 60267-1-PBS)

    IF Staining of MCF-7 using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAX antibody (60267-1-Ig, Clone: 4G5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BAX Monoclonal antibody, PBS Only

BAX Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60267-1-PBS

产品说明书

CloneNo. 4G5E8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

4G5E8, Apoptosis regulator BAX, Bcl2-L-4, Bcl-2-like protein 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BAX Ready-To-Use IHC Kit. BAX primary antibody included.

产品信息

60267-1-PBS targets BAX in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21068

Product name: Recombinant human BAX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-192 aa of BC014175

Sequence: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2-associated X protein
别名 4G5E8, Apoptosis regulator BAX, Bcl2-L-4, Bcl-2-like protein 4
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC014175
基因名称 BAX
Gene ID (NCBI) 581
RRIDAB_2848213
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ07812
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).