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验证数据展示

  • WB analysis using 17317-1-AP (same clone as 17317-1-PBS)

    WB analysis using 17317-1-AP (same clone as 17317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17317-1-AP (BAT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17317-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17317-1-AP (same clone as 17317-1-PBS)

    IHC staining of mouse brain using 17317-1-AP (same clone as 17317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17317-1-AP (BAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17317-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17317-1-AP (same clone as 17317-1-PBS)

    IHC staining of mouse brain using 17317-1-AP (same clone as 17317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17317-1-AP (BAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17317-1-PBS in a different storage buffer formulation.

BAT5 Polyclonal antibody, PBS Only

BAT5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17317-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

ABHD16A, Alpha/beta hydrolase domain-containing protein 16A, EC:3.1.-.-, EC:3.1.1.23, G5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17317-1-PBS targets BAT5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11287

Product name: Recombinant human BAT5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 209-558 aa of BC031839

Sequence: SYLVAHTLGRRMLYPGSVYLLQKALMPVLLQGQARLVEECNGRRAKLLACDGNEIDTMFVDRRGTAEPQGQKLVICCEGNAGFYEVGCVSTPLEAGYSVLGWNHPGFAGSTGVPFPQNEANAMDVVVQFAIHRLGFQPQDIIIYAWSIGGFTATWAAMSYPDVSAMILDASFDDLVPLALKVMPDSWRGLVTRTVRQHLNLNNAEQLCRYQGPVLLIRRTKDEIITTTVPEDIMSNRGNDLLLKLLQHRYPRVMAEEGLRVVRQWLEASSQLEEASIYSRWEVEEDWCLSVLRSYQAEHGPDFPWSVGEDMSADGRRQLALFLARKHLHNFEATHCTPLPAQNFQMPWHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HLA-B associated transcript 5
别名 ABHD16A, Alpha/beta hydrolase domain-containing protein 16A, EC:3.1.-.-, EC:3.1.1.23, G5
计算分子量 558 aa, 63 kDa
观测分子量59~63 KdA
GenBank蛋白编号BC031839
基因名称 BAT5
Gene ID (NCBI) 7920
RRIDAB_3741915
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95870
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human lymphocyte antigen B-associated transcript 5 (BAT5, also known as ABHD16A) is a 558 residue (63 kDa) protein encoded by 20 exons located on chromosome 6p21.33. BAT5 belongs to the α/β-hydrolase domain (ABHD) containing family of metabolic serine hydrolases and possesses both an esterase catalytic triad and an acyltransferase domain (PMID: 25290914). BAT5 not only participates in lipid metabolism but is also involved in the regulation of inflammation and immunity. BAT5 is predicted to be an integral membrane protein with highest mRNA transcript levels in mouse tissues found in testis, heart, muscle, and brain (PMID: 23328280).

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