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验证数据展示

  • WB analysis using 31383-1-AP

    WB analysis using 31383-1-AP

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 31383-1-AP (BAT2D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 31383-1-AP

    IHC staining of human placenta using 31383-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31383-1-AP (PRRC2C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRRC2C antibody included
  • IHC staining of human stomach cancer using 31383-1-AP

    IHC staining of human stomach cancer using 31383-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31383-1-AP (PRRC2C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRRC2C antibody included
  • IHC staining of rat brain using 31383-1-AP

    IHC staining of rat brain using 31383-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31383-1-AP (PRRC2C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRRC2C antibody included
  • IHC staining of rat stomach using 31383-1-AP

    IHC staining of rat stomach using 31383-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 31383-1-AP (PRRC2C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRRC2C antibody included
  • IHC staining of rat brain using 31383-1-AP

    IHC staining of rat brain using 31383-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31383-1-AP (PRRC2C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRRC2C antibody included
  • IF Staining of HeLa using 31383-1-AP

    IF Staining of HeLa using 31383-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRRC2C antibody (31383-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PRRC2C Polyclonal antibody

PRRC2C Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31383-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

BAT2D1, BAT2 iso, BAT2L2, KIAA1096, XTP2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, K-562 cells, PC-3 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® PRRC2C Ready-To-Use IHC Kit. PRRC2C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31383-1-AP targets PRRC2C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35409

Product name: Recombinant human BAT2D1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 881-1050 aa of BC006090

Sequence: DVRPHHTDANNQSACFEAPDQKTLSAPQEERISAVESQPSRKRSVSHGSNHTQKPDEQRSEPSAGIPKVTSRCIDSKEPIERPEEKPKKEGFIRSSEGPKPEKVYKSKSETRWGPRPSSNRREEVNDRPVRRSGPIKKPVLRDMKEEREQRKEKEGEKAEKVTEKVVVKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BAT2 domain containing 1
别名 BAT2D1, BAT2 iso, BAT2L2, KIAA1096, XTP2
观测分子量450 kDa
GenBank蛋白编号BC006090
基因名称 BAT2D1
Gene ID (NCBI) 23215
RRIDAB_3742423
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y520
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRRC2C encodes a RNA-binding protein that participates in stress granule assembly and is found in the cytosol and cytoplasm. The protein is also detected in cytoplasmic stress granules and membranes, placing it in compartments associated with dynamic post-transcriptional regulation. Expression measurements show particularly high levels in brain, respiratory system, and proximal digestive tract, supporting a role in tissues with active RNA regulatory demands.

实验方案

Product Specific Protocols
IF protocol for PRRC2C antibody 31383-1-APDownload protocol
IHC protocol for PRRC2C antibody 31383-1-APDownload protocol
WB protocol for PRRC2C antibody 31383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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