验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, A549 cells, HeLa cells, fetal human brain tissue |
| Positive IHC detected in | human skin cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14880-1-AP targets BASP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6653 Product name: Recombinant human BASP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC000518 Sequence: MGGKLSKKKKGYNVNDEKAKEKDKKAEGAATEEEGTPKESEPQAAAEPAEAKEGKEKPDQDAEGKAEEKEGEKDAAAAKEEAPKAEPEKTEGAAEAKAEPPKAPEQEQAAPGPAAGGEAPKAAEAAAAPAESAAPAAGEEPSKEEGEPKKTGAPAAPAAQETKSDGAPASDSKPGSSEAAPSSKETPAATEAPSSTPKAQGPAASAEEPKPVEAPAANSDQTVTVKE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | brain abundant, membrane attached signal protein 1 |
| 别名 | 22 kDa neuronal tissue-enriched acidic protein, Brain acid soluble protein 1, CAP 23, CAP23, NAP 22 |
| 计算分子量 | 23 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC000518 |
| 基因名称 | BASP1 |
| Gene ID (NCBI) | 10409 |
| RRID | AB_3085444 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P80723 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BASP1 (brain acid-soluble protein 1), also known as NAP22 or CAP-23, is an evolutionarily conserved intrinsically disordered protein that participates in cell signaling, migration, apoptosis, and gene transcription. It is highly expressed in the nervous system and plays roles in neurodevelopment, synaptic function, and nerve regeneration. BASP1 exhibits context-dependent functions in disease: it acts as a tumor suppressor in gastric cancer by inhibiting the Wnt/β-catenin pathway and in acute myeloid leukemia, while promoting atherosclerosis via vascular smooth muscle cell proliferation and inflammation. It has emerged as a promising therapeutic target and diagnostic biomarker. (PMID: 33426068; 41415108; 33015061)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BASP1 antibody 14880-1-AP | Download protocol |
| WB protocol for BASP1 antibody 14880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Breast Cancer Res Integrated multi-omics analysis and functional validation reveal the role of TRIM2 as a potential novel biomarker in breast cancer. | ||
J Gastroenterol Hepatol m6A-Modified BASP1 Regulates IL6 Expression to Induce TAM Infiltration to Promote Gastric Cancer Progression.
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