BAK Polyclonal antibody
BAK Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, Neuro-2a cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, 4T1 cells, ROS1728 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 7 publications below |
IHC | See 1 publications below |
产品信息
29552-1-AP targets BAK in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | BAK fusion protein Ag31042 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | BCL2-antagonist/killer 1 |
Synonyms | Apoptosis regulator BAK, BAK, BAK LIKE, BAK1, CDN1 |
Calculated Molecular Weight | 23 kDa |
Observed Molecular Weight | 23 kDa |
GenBank Accession Number | NM_001188 |
Gene Symbol | BAK1 |
Gene ID (NCBI) | 578 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16611 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for BAK antibody 29552-1-AP | Download protocol |
IHC protocol for BAK antibody 29552-1-AP | Download protocol |
IF protocol for BAK antibody 29552-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Apoptosis Investigation of GPR143 as a promising novel marker for the progression of skin cutaneous melanoma through bioinformatic analyses and cell experiments | ||
Life Sci A novel antimycin analogue antimycin A2c, derived from marine Streptomyces sp., suppresses HeLa cells via disrupting mitochondrial function and depleting HPV oncoproteins E6/E7 | ||
Eur J Pharmacol Platycodin D induces apoptotic cell death through PI3K/AKT and MAPK/ERK pathways and synergizes with venetoclax in acute myeloid leukemia | ||
Animal Model Exp Med FOXK1 promotes hormonally responsive breast carcinogenesis by suppressing apoptosis | ||
Chem Biol Interact Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65 | ||