验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
24836-1-AP targets BAIAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag21219 Product name: Recombinant human BAIAP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1056-1187 aa of BC104966 Sequence: PPHLFPLVRSQRTQVKTRTLHPVYDELFYFSVPAEACRRRAACVLFTVMDHDWLSTNDFAGEAALGLGGVTGVARPQVGGGARAGQPVTLHLCRPRAQVRSALRRLEGRTSKEAQEFVKKLKELEKCMEADP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BAI1-associated protein 3 |
| 别名 | BAI1 associated protein 3, BAI1-associated protein 3, BAP3, KIAA0734 |
| 计算分子量 | 1187 aa, 132 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC104966 |
| 基因名称 | BAIAP3 |
| Gene ID (NCBI) | 8938 |
| RRID | AB_2879751 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94812 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bai1 associated protein 3 (BAIAP3) is also named as BAP3 and KIAA0734, and belongs to the unc-13 family. It has the function of retrograde transport of endosomes to the Golgi apparatus. BAIAP3 may regulate behavior and food intake by controlling calcium-stimulated exocytosis of neurotransmitters including NPY and serotonin and hormones like insulin (PMID:286260000).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for BAIAP3 antibody 24836-1-AP | Download protocol |
| IHC protocol for BAIAP3 antibody 24836-1-AP | Download protocol |
| WB protocol for BAIAP3 antibody 24836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Integrated analysis of microRNA and mRNA expression profiling identifies BAIAP3 as a novel target of dysregulated hsa-miR-1972 in age-related white matter lesions. | ||
J Affect Disord Label-free proteomics differences in the dorsolateral prefrontal cortex between bipolar disorder patients with and without psychosis. |
