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验证数据展示

  • WB analysis using 86248-1-RR

    WB analysis using 86248-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86248-1-RR (BAG5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 86248-1-RR

    IF Staining of MCF-7 using 86248-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAG5 antibody (86248-1-RR, Clone: 250877D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86248-1-RR

    Affinity and Kinetic Characterization of 86248-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86248-1-RR against Human BAG5 were performed. The affinity constant is 0.286 nM.

BAG5 Recombinant monoclonal antibody

BAG5 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86248-1-RR

产品说明书

CloneNo. 250877D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

BAG 5, BAG-5, Bcl-2-associated athanogene 5, KIAA0873

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, MCF-7 cells, A549 cells, mouse brain tissue, rat brain tissue, mouse lung tissue, rat lung tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy BAG5 Ready-To-Use IHC Kit. BAG5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86248-1-RR targets BAG5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag24320

Product name: Recombinant human BAG5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 46-394 aa of BC044216

Sequence: NQHPSISRLQEIQKEVKSVEQQVIGFSGLSDDKNYKKLERILTKQLFEIDSVDTEGKGDIQQARKRAAQETERLLKELEQNANHPHRIEIQNIFEEAQSLVREKIVPFYNGGNCVTDEFEEGIQDIILRLTHVKTGGKISLRKARYHTLTKICAVQEIIEDCMKKQPSLPLSEDAHPSVAKINFVMCEVNKARGVLIALLMGVNNNETCRHLSCVLSGLIADLDALDVCGRTEIRNYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQNPSELYLSSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKEALE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-associated athanogene 5
别名 BAG 5, BAG-5, Bcl-2-associated athanogene 5, KIAA0873
计算分子量 51 kDa
观测分子量 48-51 kDa
GenBank蛋白编号BC044216
基因名称 BAG5
Gene ID (NCBI) 9529
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UL15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for BAG5 antibody 86248-1-RRDownload protocol
WB protocol for BAG5 antibody 86248-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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