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SODD/BAG4 Polyclonal antibody

SODD/BAG4 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 13913-1-AP

Print datasheet

Synonyms

BAG 4, BAG4, Silencer of death domains, SODD

验证数据展示

  • WB analysis of K-562 using 13913-1-AP

    WB analysis of K-562 using 13913-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13913-1-AP

    IP experiment of K-562 using 13913-1-AP

    IP result of anti-SODD/BAG4 (IP:13913-1-AP, 4ug; Detection:13913-1-AP 1:500) with K-562 cells lysate 2800ug.

  • IF Staining of MCF-7 using 13913-1-AP

    IF Staining of MCF-7 using 13913-1-AP

    Immunofluorescent analysis of MCF-7 cells using 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13913-1-AP targets SODD/BAG4 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited ReactivityArtemia sinica
Immunogen SODD/BAG4 fusion protein Ag4905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-associated athanogene 4
Synonyms BAG 4, BAG4, Silencer of death domains, SODD
Calculated Molecular Weight 457 aa, 50 kDa
Observed Molecular Weight 60 kDa, 66-70 kDa
GenBank Accession NumberBC038505
Gene Symbol BAG4
Gene ID (NCBI) 9530
RRIDAB_2243308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).

实验方案

Product Specific Protocols
WB protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
IF protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
IP protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
Artemia sinicaWB

Gene

Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.

Authors - Mengchen Zhang
View Article
WB

Nat Commun

Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila

Authors - Alexandra E. Perlegos
View Article