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BAG1S/1M/1L Polyclonal antibody

BAG1S/1M/1L Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16150-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

BAG1, BAG 1, HAP, Bcl-2-associated athanogene 1, BAG1L speicfic

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, Jurkat cells, mouse liver tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16150-1-AP targets BAG1S/1M/1L in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2-associated athanogene
别名 BAG1, BAG 1, HAP, Bcl-2-associated athanogene 1, BAG1L speicfic
计算分子量 26 kDa
观测分子量 46-50 kDa, 41-46 kDa, 25-33 kDa
GenBank蛋白编号NM_004323
基因名称 BAG1
Gene ID (NCBI) 573
RRIDAB_2062288
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99933
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG1 has been identified that modulates gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M, and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recognizes three isoforms of BAG1, includes GAG1L, BAG1M, and BAG1S.

实验方案

Product Specific Protocols
WB protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
IHC protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
IP protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Tumour Biol

Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro.

Authors - Wenbai Huang
  • KD Validated
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