验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27635-1-AP targets BACH2 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag26526 Product name: Recombinant human BACH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 136-235 aa of NM_001170794 Sequence: MSEDGLFVCRKDAACQRPHEDCENSAGEEEDEEEETMDSETAKMACPRDQMLPEPISFEAAAIPVAEKEEALLPEPDVPTDTKESSEKDALTQYPRYKKYQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BTB and CNC homology 1, basic leucine zipper transcription factor 2 |
| 别名 | BACH2, BTB and CNC homolog 2 |
| 计算分子量 | 93 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | NM_001170794 |
| 基因名称 | BACH2 |
| Gene ID (NCBI) | 60468 |
| RRID | AB_2880934 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYV9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BACH2, also named as Transcription regulator protein BACH2, is a 841 amino acid protein, which is widely expressed within the B-lymphoid lineage, except in plasma cells. BACH2 contains an N-terminal BTB domain involved in its transcriptional function, but instead of having zinc fingers at the C-terminus, it binds to DNA through a basic leucine zipper motif. BACH2 binds DNA consensus sequences, termed MARE motifs, by forming a heterodimer with small leucine zipper MAF proteins such as MAFK, MAFG, and MAFF. The calculated molecular weight of BACH2 is 92 kDa, but the post-modifiction of BACH2 is about 120-130 kDa. (PMID: 24277074 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BACH2 antibody 27635-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Class I HDAC inhibitors enhance antitumor efficacy and persistence of CAR-T cells by activation of the Wnt pathway | ||
J Invest Dermatol Extracellular vesicle-derived mir-148a-3p promotes plasmablast differentiation in bullous pemphigoid via BACH2 repression and mTOR activation. | ||
Antioxid Redox Signal BACH2 Exacerbates Bronchopulmonary Dysplasia by Promoting NCOA4-Dependent Ferritinophagy via Transcriptional Activation of JUN. | ||
Antioxid Redox Signal Zinc Protoporphyrin Functions as a Ferroptosis Inducer to Activate Heme-BACH Axis and Potently Suppress IDH1-Mutant Gliomas. | ||
World J Surg Oncol Prognostic value of circadian rhythm-associated genes in breast cancer | ||
FEBS Open Bio Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus.
|
