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验证数据展示

  • WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    WB analysis of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • WB analysis using 12807-1-AP (same clone as 12807-1-PBS)

    WB analysis using 12807-1-AP (same clone as 12807-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    IP experiment of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    IP result of anti-BACE1 (IP:12807-1-AP, 3ug; Detection:12807-1-AP 1:500) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    IHC staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    IF Staining of mouse brain using 12807-1-AP (same clone as 12807-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BACE1 antibody (12807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12807-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BACE1 Polyclonal antibody, PBS Only

BACE1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 12807-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, zebrafish

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

ASP2, ASP21, Aspartyl protease 2, Beta secretase 1, Beta-secretase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12807-1-PBS targets BACE1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, zebrafish
免疫原

CatNo: Ag3559

Product name: Recombinant human BACE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-457 aa of BC036084

Sequence: VRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-site APP-cleaving enzyme 1
别名 ASP2, ASP21, Aspartyl protease 2, Beta secretase 1, Beta-secretase 1
计算分子量 501 aa, 56 kDa
观测分子量 70-75 kDa, 42-56 kDa
GenBank蛋白编号BC036084
基因名称 BACE1
Gene ID (NCBI) 23621
RRIDAB_10638760
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56817
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).

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