• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82850-1-RR

    WB analysis using 82850-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82850-1-RR (B7‑H4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 82850-1-RR

    IHC staining of human ovary cancer using 82850-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82850-1-RR (B7-H4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VTCN1 antibody included
  • IHC staining of human ovary cancer using 82850-1-RR

    IHC staining of human ovary cancer using 82850-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82850-1-RR (B7-H4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VTCN1 antibody included

B7-H4 Recombinant antibody

B7-H4 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82850-1-RR

产品说明书

CloneNo. 4C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

VTCN1, B7‑H4, B7H4, B7h.5, B7 homolog 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, SKOV-3 cells, BGC-823 cells, SK-BR-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VTCN1 Ready-To-Use IHC Kit. VTCN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82850-1-RR targets B7-H4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 V-set domain containing T cell activation inhibitor 1
别名 VTCN1, B7‑H4, B7H4, B7h.5, B7 homolog 4
计算分子量282 aa, 31 kDa
观测分子量75 kDa
GenBank蛋白编号NM_024626
基因名称 B7-H4
Gene ID (NCBI) 79679
RRIDAB_3670576
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z7D3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B7-H4, also named VTCN1, B7X, or B7S1, is a single-pass type I membrane protein, which contains two immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7-H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7-H4 is over-expressed in many cancers, including breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.

实验方案

Product Specific Protocols
WB protocol for B7-H4 antibody 82850-1-RRDownload protocol
IHC protocol for B7-H4 antibody 82850-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols