验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87222-2-RR targets B4GALT6 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag13686 Product name: Recombinant human B4GALT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-382 aa of BC074884 Sequence: MSVLRRMMRVSNRSLLAFIFFFSLSSSCLYFIYVAPGIANTYLFMVQARGIMLRENVKTIGHMIRLYTNKNSTLNGTDYPEGNNSSDYLVQTTTYLPENFTYSPYLPCPEKLPYMRGFLNVNVSEVSFDEIHQLFSKDLDIEPGGHWRPKDCKPRWKVAVLIPFRNRHEHLPIFFLHLIPMLQKQRLEFAFYVIEQTGTQPFNRAMLFNVGFKEAMKDSVWDCVIFHDVDHLPENDRNYYGCGEMPRHFAAKLDKYMYILPYKEFFGGVSGLTVEQFRKINGFPNAFWGWGGEDDDLWNRVHYAGYNVTRPEGDLGKYKSIPHHHRGEVQFLGRYKLLRYSKERQYIDGLNNLIYRPKILVDRLYTNISVNLMPELAPIEDY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6 |
| 别名 | b4Gal-T6, Beta-1,4-galactosyltransferase 6, Beta-1,4-GalTase 6, Beta4Gal-T6, EC:2.4.1.- |
| 计算分子量 | 382 aa, 45 kDa |
| 观测分子量 | 45-60 kDa |
| GenBank蛋白编号 | BC074884 |
| 基因名称 | B4GALT6 |
| Gene ID (NCBI) | 9331 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UBX8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
B4GALT6 encodes for a type II membrane-bound glycoprotein, which plays a crucial role in lactosylceramide synthesis by transferring galactose from UDP-galactose to glucosylceramide. B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636). Abnormal interactions of glycosyltransferases/glycosidases such as B4GALT6 can cause imbalances in key glycosylation modifications, thereby driving the disease process through a triple cascade reaction (PMID: 41112602).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for B4GALT6 antibody 87222-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
