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验证数据展示

  • WB analysis of HEK-293 using 20148-1-AP (same clone as 20148-1-PBS)

    WB analysis of HEK-293 using 20148-1-AP (same clone as 20148-1-PBS)

    WB result of B4GALT6 antibody (20148-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B4GALT6 transfected HEK-293 cells. This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 20148-1-AP (same clone as 20148-1-PBS)

    IHC staining of human colon using 20148-1-AP (same clone as 20148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 20148-1-AP (same clone as 20148-1-PBS)

    IHC staining of human colon using 20148-1-AP (same clone as 20148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20148-1-AP (same clone as 20148-1-PBS)

    IF Staining of HeLa using 20148-1-AP (same clone as 20148-1-PBS)

    Immunofluorescent analysis of Hela cells, using B4GALT6 antibody 20148-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 20148-1-AP (same clone as 20148-1-PBS)

    WB analysis of mouse heart using 20148-1-AP (same clone as 20148-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20148-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

B4GALT6 Polyclonal antibody, PBS Only

B4GALT6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20148-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20148-1-PBS targets B4GALT6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13686

Product name: Recombinant human B4GALT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-382 aa of BC074884

Sequence: MSVLRRMMRVSNRSLLAFIFFFSLSSSCLYFIYVAPGIANTYLFMVQARGIMLRENVKTIGHMIRLYTNKNSTLNGTDYPEGNNSSDYLVQTTTYLPENFTYSPYLPCPEKLPYMRGFLNVNVSEVSFDEIHQLFSKDLDIEPGGHWRPKDCKPRWKVAVLIPFRNRHEHLPIFFLHLIPMLQKQRLEFAFYVIEQTGTQPFNRAMLFNVGFKEAMKDSVWDCVIFHDVDHLPENDRNYYGCGEMPRHFAAKLDKYMYILPYKEFFGGVSGLTVEQFRKINGFPNAFWGWGGEDDDLWNRVHYAGYNVTRPEGDLGKYKSIPHHHRGEVQFLGRYKLLRYSKERQYIDGLNNLIYRPKILVDRLYTNISVNLMPELAPIEDY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
别名
计算分子量 382 aa, 45 kDa
观测分子量 45-60 kDa
GenBank蛋白编号BC074884
基因名称 B4GALT6
Gene ID (NCBI) 9331
RRIDAB_10665357
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBX8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta-1,4-galactosyltransferase 6 (B4GALT6) is a member of the beta 4-galactosyltransferase family that catalyzes the biosynthesis of glycosphingolipids in UDP-dependent manner (PMID: 23744354). B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636).

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