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验证数据展示

  • WB analysis using 17846-1-AP

    WB analysis using 17846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17846-1-AP (B4GALT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17846-1-AP

    WB analysis using 17846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17846-1-AP (B4GALT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

B4GALT5 Polyclonal antibody

B4GALT5 Polyclonal Antibody for WB, ELISA
Cat No. 17846-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

b4Gal-T5, Beta 1,4 GalTase 5, Beta-1,4-galactosyltransferase 5, Beta-1,4-GalT II, Beta-1,4-GalTase 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17846-1-AP targets B4GALT5 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12267

Product name: Recombinant human B4GALT5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 44-388 aa of BC074873

Sequence: FMMQAQGILIRDNVRTIGAQVYEQVLRSAYAKRNSSVNDSDYPLDLNHSETFLQTTTFLPEDFTYFANHTCPERLPSMKGPIDINMSEIGMDYIHELFSKDPTIKLGGHWKPSDCMPRWKVAILIPFRNRHEHLPVLFRHLLPMLQRQRLQFAFYVVEQVGTQPFNRAMLFNVGFQEAMKDLDWDCLIFHDVDHIPESDRNYYGCGQMPRHFATKLDKYMYLLPYTEFFGGVSGLTVEQFRKINGFPNAFWGWGGEDDDLWNRVQNAGYSVSRPEGDTGKYKSIPHHHRGEVQFLGRYALLRKSKERQGLDGLNNLNYFANITYDALYKNITVNLTPELAQVNEY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
别名 b4Gal-T5, Beta 1,4 GalTase 5, Beta-1,4-galactosyltransferase 5, Beta-1,4-GalT II, Beta-1,4-GalTase 5
计算分子量 388 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC074873
基因名称 B4GALT5
Gene ID (NCBI) 9334
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43286
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B4GALT5 (beta-1,4-galactosyltransferase 5) catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer). B4GALT5 is present on the cell surface and located in the Golgi complex, similar to other glycosyltransferases that function as adhesion molecules involved in matrix interactions, cell spreading and migration, and signal transduction cascades (PMID: 35410157).

实验方案

Product Specific Protocols
WB protocol for B4GALT5 antibody 17846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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