验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, mouse kidney tissue, mouse lung tissue, mouse thymus tissue, rat kidney tissue, rat thymus tissue, mouse cerebellum tissue |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30478-1-AP targets B4GALT4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag32337 Product name: Recombinant human B4GALT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 39-142 aa of BC004523 Sequence: QEIPKAKEFMANFHKTLILGKGKTLTNEASTKKVELDNCPSVSPYLRGQSKLIFKPDLTLEEVQAENPKVSRGRYRPEECKALQRVAILVPHRNREKHLMYLLE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 |
| 别名 | beta4Gal T4, Beta-1,4-GalTase 4, Beta-1,4-galactosyltransferase 4, Beta 1,4 GalTase 4, b4Gal-T4 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 34-40 kDa |
| GenBank蛋白编号 | BC004523 |
| 基因名称 | B4GALT4 |
| Gene ID (NCBI) | 8702 |
| RRID | AB_3086331 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60513 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for B4GALT4 antibody 30478-1-AP | Download protocol |
| WB protocol for B4GALT4 antibody 30478-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
