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验证数据展示

  • WB analysis using 15200-1-AP

    WB analysis using 15200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15200-1-AP (B4GALT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 15200-1-AP

    WB analysis of mouse skeletal muscle using 15200-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15200-1-AP (B4GALT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15200-1-AP

    IHC staining of human kidney using 15200-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15200-1-AP (B4GALT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15200-1-AP

    IHC staining of human kidney using 15200-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15200-1-AP (B4GALT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 15200-1-AP

    IF Staining of A549 using 15200-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using B4GALT4 antibody (15200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

B4GALT4 Polyclonal antibody

B4GALT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15200-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

beta4Gal T4, Beta-1,4-GalTase 4, Beta-1,4-galactosyltransferase 4, Beta 1,4 GalTase 4, b4Gal-T4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCaco-2 cells, mouse skeletal muscle tissue, PC-3 cells, HaCaT cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15200-1-AP targets B4GALT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7348

Product name: Recombinant human B4GALT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-344 aa of BC004523

Sequence: GAIQEIPKAKEFMANFHKTLILGKGKTLTNEASTKKVELDNCPSVSPYLRGQSKLIFKPDLTLEEVQAENPKVSRGRYRPEECKALQRVAILVPHRNREKHLMYLLEHLHPFLQRQQLDYGIYVIHQAEGKKFNRAKLLNVGYLEALKEENWDCFIFHDVDLVPENDFNLYKCEEHPKHLVVGRNSTGYRLRYSGYFGGVTALSREQFFKVNGFSNNYWGWGGEDDDLRLRVELQRMKISRPLPEVGKYTMVFHTRDKGNEVNAERMKLLHQVSRVWRTDGLSSCSYKLVSVEHNPLYINITVDFWFGA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
别名 beta4Gal T4, Beta-1,4-GalTase 4, Beta-1,4-galactosyltransferase 4, Beta 1,4 GalTase 4, b4Gal-T4
计算分子量 40 kDa
观测分子量 42 kDa
GenBank蛋白编号BC004523
基因名称 B4GALT4
Gene ID (NCBI) 8702
RRIDAB_2290074
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60513
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for B4GALT4 antibody 15200-1-APDownload protocol
IHC protocol for B4GALT4 antibody 15200-1-APDownload protocol
WB protocol for B4GALT4 antibody 15200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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