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验证数据展示

  • WB analysis using 11041-1-AP (same clone as 11041-1-PBS)

    WB analysis using 11041-1-AP (same clone as 11041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11041-1-AP (B4GALT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

    IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

    IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 11041-1-AP (same clone as 11041-1-PBS)

    IF Staining of A431 using 11041-1-AP (same clone as 11041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 11041-1-AP (B4GALT3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 11041-1-AP (same clone as 11041-1-PBS)

    IF Staining of MCF-7 using 11041-1-AP (same clone as 11041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using B4GALT3 antibody (11041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

B4GALT3 Polyclonal antibody, PBS Only

B4GALT3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11041-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

b4Gal T3, b4Gal-T3, Beta 1,4 GalTase 3, Beta-1,4-galactosyltransferase 3, Beta-1,4-GalTase 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11041-1-PBS targets B4GALT3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1487

Product name: Recombinant human B4GALT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-321 aa of BC009985

Sequence: MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDQGPTFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
别名 b4Gal T3, b4Gal-T3, Beta 1,4 GalTase 3, Beta-1,4-galactosyltransferase 3, Beta-1,4-GalTase 3
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC009985
基因名称 B4GALT3
Gene ID (NCBI) 8703
RRIDAB_2290072
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60512
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

B4GALT3 is an enzyme responsible for the generation of poly-N-acetyllactosamine. The B4GALTs transfer galactose from UDP-Gal to N-acetylglucosamine (GlcNAc)-terminated oligosaccharides on N-glycan, O-glycan, or glycolipid to form N-acetyllactosamin (PMID: 23444218). B4GALT3 has been found to regulate cancer cell invasion (PMID: 25659296). B4GALT3 knockdown increased cell migration, invasion and the activation of β1 integrin and its downstream signaling (PMID: 24403309).

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