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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 55448-1-AP

    WB analysis using 55448-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55448-1-AP (B4GALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

B4GALT1 Polyclonal antibody

B4GALT1 Polyclonal Antibody for WB, ELISA
Cat No. 55448-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

GTB, GT1, GGTB2, beta4Gal T1, Beta 1,4 GalTase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, mouse small intestine tissue, mouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55448-1-AP targets B4GALT1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
别名 GTB, GT1, GGTB2, beta4Gal T1, Beta 1,4 GalTase 1
计算分子量 44 kDa
观测分子量40-44 kDa
GenBank蛋白编号NM_001497
基因名称 B4GALT1
Gene ID (NCBI) 2683
RRIDAB_3670253
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15291
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B4GALT1 is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. B4GALT1 is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. B4GALT1 has two isoforms with molecular weights of 44 kda and 43 kda that are localized to the cell surface and Golgi apparatus, respectively.

实验方案

Product Specific Protocols
WB protocol for B4GALT1 antibody 55448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols