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验证数据展示

  • IHC staining of mouse stomach using 24675-1-AP

    IHC staining of mouse stomach using 24675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24675-1-AP (B4GALNT3 antibody) at dilution of 1:200 (under 40x lens)..

  • WB analysis of Transfected HEK-293 using 24675-1-AP

    WB analysis of Transfected HEK-293 using 24675-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 24675-1-AP (B4GALNT3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IHC staining of mouse stomach using 24675-1-AP

    IHC staining of mouse stomach using 24675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24675-1-AP (B4GALNT3 antibody) at dilution of 1:200 (under 10x lens)..

B4GALNT3 Polyclonal antibody

B4GALNT3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24675-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

B4GalNac T3, B4GALNT3, Beta4GalNAc T3, Beta4GalNAcT3, NGalNAc T2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTransfected HEK-293 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24675-1-AP targets B4GALNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 B4GALNT3 fusion protein Ag20342 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-1,4-N-acetyl-galactosaminyl transferase 3
别名 B4GalNac T3, B4GALNT3, Beta4GalNAc T3, Beta4GalNAcT3, NGalNAc T2
计算分子量 998 aa, 115 kDa
观测分子量100 kDa
GenBank蛋白编号BC156653
基因名称 B4GALNT3
Gene ID (NCBI) 283358
RRIDAB_3085748
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6L9W6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B4GALNT3 (β1,4-N-acetylgalactosaminyltransferase III) has in vitro activity to promote the synthesis of LacdiNAc, an important structure found on glycoproteins expressed by neurons. B4GALNT3 was highly expressed in either differentiated NB or mature ganglion cells (PMID: 21741930). B4GALNT3 is predominantly expressed in the stomach, colon, and testis (PMID: 32267274).

实验方案

Product Specific Protocols
WB protocol for B4GALNT3 antibody 24675-1-APDownload protocol
IHC protocol for B4GALNT3 antibody 24675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols