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B4GALNT3 Polyclonal antibody

B4GALNT3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24675-1-AP

Print datasheet

Synonyms

B4GalNac T3, B4GALNT3, Beta4GalNAc T3, Beta4GalNAcT3, NGalNAc T2

验证数据展示

  • IHC staining of mouse stomach using 24675-1-AP

    IHC staining of mouse stomach using 24675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24675-1-AP (B4GALNT3 antibody) at dilution of 1:200 (under 40x lens)..

  • WB analysis of Transfected HEK-293 using 24675-1-AP

    WB analysis of Transfected HEK-293 using 24675-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 24675-1-AP (B4GALNT3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IHC staining of mouse stomach using 24675-1-AP

    IHC staining of mouse stomach using 24675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24675-1-AP (B4GALNT3 antibody) at dilution of 1:200 (under 10x lens)..

经过测试的应用

Positive WB detected inTransfected HEK-293 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24675-1-AP targets B4GALNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen B4GALNT3 fusion protein Ag20342 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name beta-1,4-N-acetyl-galactosaminyl transferase 3
Synonyms B4GalNac T3, B4GALNT3, Beta4GalNAc T3, Beta4GalNAcT3, NGalNAc T2
Calculated Molecular Weight 998 aa, 115 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC156653
Gene Symbol B4GALNT3
Gene ID (NCBI) 283358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6L9W6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B4GALNT3 (β1,4-N-acetylgalactosaminyltransferase III) has in vitro activity to promote the synthesis of LacdiNAc, an important structure found on glycoproteins expressed by neurons. B4GALNT3 was highly expressed in either differentiated NB or mature ganglion cells (PMID: 21741930). B4GALNT3 is predominantly expressed in the stomach, colon, and testis (PMID: 32267274).

实验方案

Product Specific Protocols
WB protocol for B4GALNT3 antibody 24675-1-APDownload protocol
IHC protocol for B4GALNT3 antibody 24675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols