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B4GALNT2 Polyclonal antibody

B4GALNT2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24024-1-AP

Print datasheet

Synonyms

B4GALGT2, B4GALNT2, B4GALT, Cad, GALGT2, SD, Sda

验证数据展示

  • WB analysis of Jurkat using 24024-1-AP

    WB analysis of Jurkat using 24024-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24024-1-AP

    IHC staining of human colon cancer using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 24024-1-AP

    IHC staining of human colon cancer using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24024-1-AP is B4GALNT2 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen B4GALNT2 fusion protein Ag21166 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name beta-1,4-N-acetyl-galactosaminyl transferase 2
Synonyms B4GALGT2, B4GALNT2, B4GALT, Cad, GALGT2, SD, Sda
Calculated Molecular Weight 566 aa, 63 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC113677
Gene Symbol B4GALNT2
Gene ID (NCBI) 124872
RRIDAB_2879405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8NHY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B4GALNT2, also named as GALGT2, catalyzes the last step in the biosynthesis of the human Sd(a) antigen, which is found on more than 90% of Caucasian red blood cells. It also catalyzes the last step in the biosynthesis of the Cad antigen, which is related both serologically and biochemically to the Sd(a) antigen. B4GALNT2 transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. This antibody detects the 55-65 kDa B4GALNT2 protein.

实验方案

Product Specific Protocols
WB protocol for B4GALNT2 antibody 24024-1-APDownload protocol
IHC protocol for B4GALNT2 antibody 24024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols