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B3GNT5 Polyclonal antibody

B3GNT5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20422-1-AP

Print datasheet

Synonyms

B3GN T5, B3GNT5, Beta 1,3 Gn T5, beta3Gn T5, BGnT 5, Lactotriaosylceramide synthase, Lc(3)Cer synthase, Lc3 synthase

验证数据展示

  • WB analysis of mouse lung using 20422-1-AP

    WB analysis of mouse lung using 20422-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20422-1-AP (B3GNT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 20422-1-AP

    IHC staining of human lung using 20422-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20422-1-AP (B3GNT5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 20422-1-AP

    IHC staining of human lung using 20422-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20422-1-AP (B3GNT5 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20422-1-AP is B3GNT5 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen B3GNT5 fusion protein Ag14246 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Synonyms B3GN T5, B3GNT5, Beta 1,3 Gn T5, beta3Gn T5, BGnT 5, Lactotriaosylceramide synthase, Lc(3)Cer synthase, Lc3 synthase
Calculated Molecular Weight 378 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC028058
Gene Symbol B3GNT5
Gene ID (NCBI) 84002
RRIDAB_10694280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYG0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for B3GNT5 antibody 20422-1-APDownload protocol
IHC protocol for B3GNT5 antibody 20422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Cell Mol Med

MiR-30a-5p inhibits proliferation and metastasis of hydatidiform mole by regulating B3GNT5 through ERK/AKT pathways.

Authors - Zhenzhen Guo
View Article
humanWB

Cell Rep

Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins

Authors - Jicheng Yang
View Article