验证数据展示
经过测试的应用
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below | 
产品信息
13490-1-AP targets B3GALT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description | 
| 文献引用应用 | WB | 
| 经测试反应性 | human, mouse, rat | 
| 文献引用反应性 | human, mouse | 
| 免疫原 | CatNo: Ag4312 Product name: Recombinant human B3GALT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 83-378 aa of BC032574 Sequence: NLNQRNAIRASWGGLREARGLRVQTLFLLGEPNAQHPVWGSQGSDLASESAAQGDILQAAFQDSYRNLTLKTLSGLNWAEKHCPMARYVLKTDDDVYVNVPELVSELVLRGGRWGQWERSTEPQREAEQEGGQVLHSEEVPLLYLGRVHWRVNPSRTPGGRHRVSEEQWPHTWGPFPPYASGTGYVLSASAVQLILKVASRAPLLPLEDVFVGVSARRGGLAPTQCVKLAGATHYPLDRCCYGKFLLTSHRLDPWKMQEAWKLVGGSDGERTAPFCSWFQGVLGILRCRAIAWLQS种属同源性预测 | 
| 宿主/亚型 | Rabbit / IgG | 
| 抗体类别 | Polyclonal | 
| 产品类型 | Antibody | 
| 全称 | UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4 | 
| 别名 | b3Gal T4, b3Gal-T4, Beta 1,3 GalTase 4, BETA 3 GALT4, Beta-1,3-galactosyltransferase 4 | 
| 计算分子量 | 378 aa, 42 kDa | 
| GenBank蛋白编号 | BC032574 | 
| 基因名称 | B3GALT4 | 
| Gene ID (NCBI) | 8705 | 
| RRID | AB_2063054 | 
| 偶联类型 | Unconjugated | 
| 形式 | Liquid | 
| 纯化方式 | Antigen affinity purification | 
| UNIPROT ID | O96024 | 
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for B3GALT4 antibody 13490-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
发表文章
| Species | Application | Title | 
|---|---|---|
| FASEB J Functional role of gangliotetraosylceramide in epithelial-to-mesenchymal transition process induced by hypoxia and by TGF-{beta}. | ||
| Sci Rep TNFα-signal and cAMP-mediated signals oppositely regulate melanoma- associated ganglioside GD3 synthase gene in human melanocytes. | 


