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验证数据展示

  • WB analysis of mouse brain using 14177-1-AP

    WB analysis of mouse brain using 14177-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14177-1-AP (B3GALT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 14177-1-AP

    IHC staining of human heart using 14177-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14177-1-AP (B3GALT2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 14177-1-AP

    IHC staining of human heart using 14177-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14177-1-AP (B3GALT2 antibody) at dilution of 1:50 (under 40x lens).

B3GALT2 Polyclonal antibody

B3GALT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14177-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

B3GALT2, Beta 1,3 GalTase 2, beta3Gal T2, BETA3GALT2, GLCT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14177-1-AP targets B3GALT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 B3GALT2 fusion protein Ag5384 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2
别名 B3GALT2, Beta 1,3 GalTase 2, beta3Gal T2, BETA3GALT2, GLCT2
计算分子量 422 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC022507
基因名称 B3GALT2
Gene ID (NCBI) 8707
RRIDAB_11182828
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43825
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for B3GALT2 antibody 14177-1-APDownload protocol
IHC protocol for B3GALT2 antibody 14177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols