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验证数据展示

  • WB analysis using 22048-1-AP

    WB analysis using 22048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse placenta using 22048-1-AP

    WB analysis of mouse placenta using 22048-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 22048-1-AP

    IHC staining of human heart using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 22048-1-AP

    IHC staining of human heart using 22048-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 40x lens).

B3GALNT1 Polyclonal antibody

B3GALNT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22048-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

b3Gal T3, B3GALNT1, B3GALT3, Beta 1,3 GalNAc T1, Beta 1,3 GalTase 3, Beta 3 Gx T3, beta3Gal T3, Beta3GalT3, galT3, Gb4Cer, GLCT3, GLOB, Globoside synthase, P1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse placenta tissue, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22048-1-AP targets B3GALNT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16976

Product name: Recombinant human B3GALNT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 43-331 aa of BC047618

Sequence: VIERVNWMYFYEYEPIYRQDFHFTLREHSNCSHQNPFLVILVTSHPSDVKARQAIRVTWGEKKSWWGYEVLTFFLLGQEAEKEDKMLALSLEDEHLLYGDIIRQDFLDTYNNLTLKTIMAFRWVTEFCPNAKYVMKTDTDVFINTGNLVKYLLNLNHSEKFFTGYPLIDNYSYRGFYQKTHISYQEYPFKVFPPYCSGLGYIMSRDLVPRIYEMMGHVKPIKFEDVYVGICLNLLKVNIHIPEDTNLFFLYRIHLDVCQLRRVIAAHGFSSKEIITFWQVMLRNTTCHY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
别名 b3Gal T3, B3GALNT1, B3GALT3, Beta 1,3 GalNAc T1, Beta 1,3 GalTase 3, Beta 3 Gx T3, beta3Gal T3, Beta3GalT3, galT3, Gb4Cer, GLCT3, GLOB, Globoside synthase, P1
计算分子量 40 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC047618
基因名称 B3GALNT1
Gene ID (NCBI) 8706
RRIDAB_11182184
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75752
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for B3GALNT1 antibody 22048-1-APDownload protocol
IHC protocol for B3GALNT1 antibody 22048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols