AuthentiKine™ Human CXCL8/IL-8 ELISA Kit
Validation Data Gallery
Product Information
KE00275 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CXCL8/IL-8 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL8/IL-8. The assay recognizes human CXCL8/IL-8. An antibody specific for CXCL8/IL-8 has been pre-coated onto the microwells. The CXCL8/IL-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human CXCL8/IL-8 is added to detect the captured human CXCL8/IL-8 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human CXCL8/IL-8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 1.56-100 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3576 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 107% | 76%-128% |
| Cell culture supernatants | 106% | 86%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 86.5 | 3.8 | 4.4 |
| 2 | 20 | 19.9 | 0.3 | 1.6 |
| 3 | 20 | 5.0 | 0.1 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 82.0 | 4.2 | 5.1 |
| 2 | 24 | 19.3 | 0.7 | 3.4 |
| 3 | 24 | 4.5 | 0.3 | 7.4 |
Background Information
Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CXCL8,IL8,IL-8,C X C motif chemokine 8,Chemokine |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Sci Rep Protective effects of a new generation of probiotic Bacteroides fragilis against colitis in vitro and in vitro | ||
Adv Sci (Weinh) Exosomes-transferred LINC00668 Contributes to Thrombosis by Promoting NETs Formation in Inflammatory Bowel Disease | ||
Heliyon Abnormal expression of CEBPB promotes the progression of renal cell carcinoma through regulating the generation of IL-6 | ||
J Cancer Matrix stiffness regulates NPC invasiveness by modulating a mechanoresponsive TRPV4-Nox4-IL-8 signaling axis | ||
Phytomedicine Tetrandrine alleviates macrophage activation syndrome after CAR-T cell therapy | ||
Int J Biol Sci Targeting the LHX1-LDB1 Complex Restores STING-dependent Senescence Surveillance and Inhibits Head and Neck Cancer Progression. |
