AuthentiKine™ Human IL-28B ELISA Kit
Validation Data Gallery
Product Information
KE00235 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-28B ELISA kit is to be used to detect and quantify protein levels of endogenous IL-28B. The assay recognizes human IL-28B. An antibody specific for IL-28B has been pre-coated onto the microwells. The IL-28B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-28B is added to detect the captured IL-28B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-28B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants , Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 282617 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 101% | 95%-112% |
| Cell culture supernatants | 111% | 98%-123% |
| Urine | 96% | 90%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 423.9 | 7.1 | 1.7 |
| 2 | 20 | 103.5 | 2.6 | 2.6 |
| 3 | 20 | 22.3 | 0.4 | 1.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 427.6 | 9.0 | 2.1 |
| 2 | 24 | 101.5 | 1.1 | 1.1 |
| 3 | 24 | 22.8 | 0.5 | 2.2 |
Background Information
IL-28B, also named as IFNL3, is a cytokine distantly related to type I interferons and the IL-10 family. IL-28B, interleukin 28A (IL-28A), and interleukin 29 (IL-29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL-10RB) and interleukin 28 receptor, alpha (IL-28RA). IL-28B was identified as a key regulator of B- and T-cell vaccine responses against influenza.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL28B, IL-28B/ IFNL3, Cytokine Zcyto22, IFN lambda 3, IFN lambda 4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
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