AuthentiKine™ Human IFN alpha 2A ELISA Kit
Validation Data Gallery
Product Information
KE00207 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN alpha 2A ELISA kit is to be used to detect and quantify protein levels of endogenous IFN alpha 2A. The assay recognizes human IFN alpha 2A. An antibody specific for IFN alpha 2A has been pre-coated onto the microwells. The IFN alpha 2A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IFN alpha 2A is added to detect the captured IFN alpha 2A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IFN alpha 2A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3440 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 96% | 85%-104% |
| Human Plasma | 98% | 82%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 238.7 | 3.5 | 1.4 |
| 2 | 20 | 55.2 | 0.8 | 1.5 |
| 3 | 20 | 13.1 | 0.6 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 231.1 | 4.4 | 1.9 |
| 2 | 24 | 53.8 | 1.0 | 1.9 |
| 3 | 24 | 13.7 | 0.7 | 5.2 |
Background Information
Human interferon alpha-2 (IFNA2), is a member of the Type I interferon cytokine family, known for its antiviral and anti-proliferative functions. Several IFN alpha2 alleles have been described, and the best known are alpha-2a and alpha-2b. IFN alpha2 binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. interferon alpha 2 is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. Interferon alpha-2 products, such as interferon alpha-2a (IFNA2) are used to treat multiple diseases such as hairy cell leukemia and hepatitis c.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IFN alpha 2, IFNA2, IFN alpha 2A, IFN alpha2, IFN-alpha 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Rep IFNα-induced BST2+ tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling |
