AuthentiKine™ Human FLT3 Ligand ELISA Kit
Sensitivity
0.15 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00197 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FLT3 Ligand ELISA kit is to be used to detect and quantify protein levels of endogenous FLT3 Ligand. The assay recognizes human FLT3 Ligand. An antibody specific for FLT3 Ligand has been pre-coated onto the microwells. The FLT3 Ligand protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for FLT3 Ligand is added to detect the captured FLT3 Ligand protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human FLT3 Ligand ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.15 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2323 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 89 | 81-105 |
| Cell culture supernatants | 89 | 76-101 |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 511.8 | 13.4 | 2.6 |
| 2 | 20 | 124.7 | 2.9 | 2.3 |
| 3 | 20 | 28.2 | 1.2 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 517.1 | 17.3 | 3.3 |
| 2 | 24 | 123.4 | 4.6 | 3.7 |
| 3 | 24 | 28.5 | 1.5 | 5.1 |
Background Information
FLT3 Ligand is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cell. FLT3 ligand is known to induce profound expansion of dendritic cells (DCs) in the peripheral lymphoid organs, and FLT3 ligand-induced DCs have an immature phenotype. Its receptor, FLT3, is expressed on myeloid, lymphoid and dendritic cell progenitors and is considered an important growth and differentiation factor for several hematopoietic lineages. Activating mutations of FLT3 are frequently found in acute myeloid leukemia (AML) patients and associated with a poor clinical prognosis. Serum FLT3 Ligand is a biomarker of progenitor cell mass and prognosis in acute myeloid leukemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
