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Species Reactivity
0
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0
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0
Conjugate
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验证数据展示

  • WB analysis using 80919-2-RR (same clone as 80919-2-PBS)

    WB analysis using 80919-2-RR (same clone as 80919-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80919-2-RR (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80919-2-PBS in a different storage buffer formulation.

  • WB analysis using 80919-2-RR (same clone as 80919-2-PBS)

    WB analysis using 80919-2-RR (same clone as 80919-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80919-2-RR (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80919-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02584-1

    Sandwich ELISA standard curve of MP02584-1

    Sandwich ELISA standard curve of MP02584-1, Human Ataxin 2 Recombinant Matched Antibody Pair - PBS only. 80919-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16470. 80919-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • Sample test of MP02584-1

    Sample test of MP02584-1

    The mean Ataxin 2 concentration was determined to be 2.25 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load.

  • IP experiment of NIH/3T3 using 80919-2-RR (same clone as 80919-2-PBS)

    IP experiment of NIH/3T3 using 80919-2-RR (same clone as 80919-2-PBS)

    IP result of anti-Ataxin 2 (IP:80919-2-RR, 4ug; Detection:80919-2-RR 1:1000) with NIH/3T3 cells lysate 1200 ug. This data was developed using the same antibody clone with 80919-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 80919-2-RR (same clone as 80919-2-PBS)

    IHC staining of mouse brain using 80919-2-RR (same clone as 80919-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80919-2-RR (Ataxin 2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80919-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 80919-2-RR (same clone as 80919-2-PBS)

    IF Staining of mouse brain using 80919-2-RR (same clone as 80919-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Ataxin 2 antibody (80919-2-RR, Clone: 250888E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80919-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80919-2-RR

    Affinity and Kinetic Characterization of 80919-2-RR

    Biolayer interferometry (BLl) kinetic assays of 80919-2-RR against Human Ataxin 2 were performed. The affinity constant is 0.153 nM.

    {{ptg:ProductFeaturesInfo}}

Ataxin 2 Recombinant monoclonal antibody, PBS Only (Capture)

Ataxin 2 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IP, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 80919-2-PBS

产品说明书

CloneNo. 250888E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IP, Sandwich ELISA, Indirect ELISA, Sample test

别名

ATXN2, ataxin2, Ataxin-2, ATX2, ATXN 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80919-2-PBS targets Ataxin 2 as part of a matched antibody pair:

MP02584-1: 80919-2-PBS capture and 80919-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, IP, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16470

Product name: Recombinant human Ataxin 2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 251-600 aa of BC114546

Sequence: GSDQRVVNGGVPWPSPCPSPSSRPPSRYQSGPNSLPPRAATPTRPPSRPPSRPSRPPSHPSAHGSPAPVSTMPKRMSSEGPPRMSPKAQRHPRNHRVSAGRGSISSGLEFVSHNPPSEAATPPVARTSPSGGTWSSVVSGVPRLSPKTHRPRSPRQNSIGNTPSGPVLASPQAGIIPTEAVAMPIPAASPTPASPASNRAVTPSSEAKDSRLQDQRQNSPAGNKENIKPNETSPSFSKAENKGISPVVSEHRKQIDDLKKFKNDFRLQPSSTSESMDQLLNKNREGEKSRDLIKDKIEPSAKDSFIENSSSNCTSGSSKPNSPSISPSILSNTEHKRGPEVTSQGVQTSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ataxin 2
别名 ATXN2, ataxin2, Ataxin-2, ATX2, ATXN 2
计算分子量 1313 aa, 140 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC114546
基因名称 ATXN2
Gene ID (NCBI) 6311
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99700
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]